G-Banded Karyotyping

Cell Line Genetics (CLG) is recognized world-wide for its expertise in cell line karyotyping analysis. Unlike most cytogenetic core facilities and commercial laboratories focused on clinical testing, CLG provides cell therapy and regenerative medicine researchers with the highest quality cytogenetics service available with guaranteed, fast turn-around time.

What is Karyotyping?
Karyotyping is an essential tool for ensuring genetic integrity, determining copy number variances (CNV), deletions and translocations (balanced and unbalanced). It is still regarded as the gold standard for copy number variance detection. Karyotyping pairs and aligns all the homologous chromosomes within a cell. From this, a cytogeneticist can analyze several forms of structural genetic aberrations.

CLG’s scientists are clinically trained and experienced in cell genomic stability analysis and structural chromosomal anomalies unique to various types of cell lines—many of which can lead to invasive cell populations and other adverse genetic events. Since the company’s inception, CLG has characterized thousands of research and therapeutic cell lines including:

  • Stem Cells (e.g., Embryonic, Induced Pluripotent, Hematopoietic)
  • Primary Cells, CAR-T Cells and NK Cells
  • Gene-Edited Cells (CRISPR-Cas9 or ZFN)
  • Multiple Species (e.g., Human, Mouse, Rat, Rhesus)

Cell Line Genetics, in partnership with Leica Biosystems, uses the latest version of the Cytovision GSL-120 image analysis and capture system. The GSL-120 is an integrated, scalable platform that allows for consistent, replicable results across all samples.

Submit Samples for Testing
  • Live cell cultures and metaphase cell pellets accepted for chromosome analysis.
  • Chromosome analysis of 20 cells per cell line.
  • High quality G-band karyotype images in publication ready format.
  • Comprehensive interpretation of results by a CLG scientist.
  • Report reviewed and signed by Clinical Certified Cytogeneticist upon request.
  • Results securely stored in CLG database.
  • 7-10 day turn around – Rush services are available.
  • Both Research Use Only (RUO) and Good Laboratory Practice (GLP) service available.
Used to:
  • As a baseline screen:
    • When cell lines are derived
    • Before the start of an experiment
    • At the conclusion of an experiment
    • Before publication
    • As part of routine cell line QC
    • As part of cell banking QC
  • When cultures show unusual growth properties
Detects:
  • Microscopic aberrations >5Mb
  • Chromosome abnormalities:
    • Aneuploidy
    • Inversions
    • Duplications/deletions
    • Translocations
Doesn’t Detect:
  • Submicroscopic aberrations <5Mb in size
Sample Requirements:
  • Live Cell Culture (T-25 Flask)
  • Cell pellets
Species:
Turnaround:
  • 7 – 10 business days RUSH services available

PROCEDURE: Mailing Live Cultures for Karyotyping

Requisition Form
Each sample must be accompanied by its own Test Requisition Form. Fill out a requisition form here.