Fluorescence in Situ Hybridization (FISH) Services

FISH is a sensitive and useful tool for detecting small populations of interphase and metaphase cells with clonal aberrations. Once a clonal aberration is identified by routine karyotyping or aCGH, FISH probes targeting the same aberration can be used on earlier passages of the cell line to determine whether earlier passages are aberration-free. FISH can be used track longitudinal cytogenetic changes in cancer cells. Because of its high degree of sensitivity, FISH can also be used to screen large numbers of cultures for recurrent chromosome aberrations and can be used to detect recurrent aberrations in cell lines that otherwise appear normal by standard cytogenetics.

What is Fluorescence in Situ Hybridization (FISH)?
FISH involves tagging DNA of interest with complementary fluorescent probes that can be documented with fluorescent microscopy.

Cell Line Genetics offers standard FISH services as well as an array of highly customizable options such as designing specific genetic probes or verification of cells that have been CRISPR-based gene edited.

Services:

  • Standard FISH services: Used to identify small emerging abnormal clones, complex chromosome aberrations, small partial trisomies, small marker chromosomes, and to refine chromosome breakpoints.
    • CLG offers FISH services for a large variety of genes and chromosome regions, including CLG StemCheckTM Probes
    • The CLG Human StemCheckTM FISH probes provide users with a quick and economic means to assess the occurrence of trisomy within their cultured Stem Cell lines.
      • The StemCheckTM FISH probes target the 4 most common trisomies (Chr 1,12,17,20) that can arise during the culturing and passaging of ESC and iPSC lines.
      • CLG’s Critical 4 (human) were identified by leveraging our library of Stem Cell Abnormalities obtained through the evaluation of more than 17,000 cultured human iPSC and ESC lines.
      • Since FISH analysis provides a cell-by-cell perspective of the overall cell population (single cell), users of the StemCheckTM FISH Probes will be able to evaluate for mixed populations.
    • CLG offers gene specific FISH probes for rapid determination of gene copy number.
      • Some genes offered include PTEN, BCL2L1, EGFR, RET, etc.
    • Complimentary aCGH verification: Confirm aCGH duplications and deletions using fluorescently labeled probes.
      • CLG uses fluorescence in situ hybridization (FISH) to confirm array comparative genomic hybridization (aCGH) findings. CLG can confirm genomic amplifications and deletions along with detecting the location of the aberrations that are larger than 40KB in size.
    • Transgene integration: Determine the chromosome band location of your inserted transgene.
      • CLG can use fluorescence in situ hybridization (FISH) to confirm the integration of transgene and determine their chromosome band location.
    • Gene editing verification
    • Custom FISH probe design, localization, and QA: Can’t find the probe you are looking for? CLG can provide specific FISH probes to meet your research need.
      • Probes for specific genes (larger than 40kb), chromosomes bands, and specific chromosome regions.
      • CLG has the FISH expertise to work with difficult research samples, including cancer and gene-edited cell lines.
Basic
Allele Table
X
Electropherogram (By Request)
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Results Archived in CLG Database
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Comprehensive Interpretation of Results
Profile Comparison Analysis
Database Cross-Referencing Analysis
DNA Extraction
Comprehensive
Allele Table
X
Electropherogram (By Request)
X
Results Archived in CLG Database
X
Comprehensive Interpretation of Results
X
Profile Comparison Analysis
X
Database Cross-Referencing Analysis
X
DNA Extraction
X
Submit Samples for Testing
  • Harvesting of live cell cultures for FISH analysis.
  • Fixed cell pellets and frozen cell pellets also accepted.
  • Interphase Nuclei analysis of 200 cells per cell line. Metaphase analysis also available.
  • High quality images in publication ready format.
  • Comprehensive interpretation of results by a CLG scientist.
  • Report signed by clinically certified cytogeneticist.
  • Results securely stored in CLG database.
  • 7-10 day turn around – Rush services are available.
  Standard FISH Services
Used to:
  • As an adjunct to G-Band Karyotyping:
    • To identify complex chromosome aberrations
    • To identify small partial trisomies
    • To identify small marker chromosomes
    • To refine chromosome breakpoints
    • To detect small emerging abnormal clones
Detects:
  • Genomic sequences of interest:
    • Duplications and deletions >40KB
    • Low level mosaicism
    • Unbalanced translocations
    • Cryptic chromosome aberrations
Doesn’t Detect:
  • Chromosome aberrations other than the probe sequence of interest
Sample Requirements:
  • Live Cell Culture (T-25 Flask)
  • Frozen Cells
Species:
  • Human
  • Mouse
  • Contact us about other species
Turnaround:
  • 7 – 10 business days
  • RUSH services available

PROCEDURE: Mailing Live Cultures For FISH

Requisition Form
Each sample must be accompanied by its own Test Requisition Form. Fill out a requisition form here.

      1. Merz, M., Jauch, A., Hielscher, T., Mai, E. K., Seckinger, A., Hose, D., Bertsch, U., Neben, K., Raab, M. S., Salwender, H., Blau, I. W., Lindemann, H. W., Schmidt-Wolf, I., Scheid, C., Haenel, M., Weisel, K., Goldschmidt, H., & Hillengass, J. (2017). Longitudinal fluorescence in situhybridization reveals cytogenetic evolution in myeloma relapsing after autologous transplantation. Haematologica, 102(8), 1432–1438. https://doi.org/10.3324/haematol.2017.168005

 

    1. Shakoori A. R. (2017). Fluorescence In Situ Hybridization (FISH) and Its Applications. Chromosome Structure and Aberrations, 343–367. https://doi.org/10.1007/978-81-322-3673-3_16