{"id":239355,"date":"2021-05-13T19:26:58","date_gmt":"2021-05-13T19:26:58","guid":{"rendered":"http:\/\/wolfson.net\/clgenetics\/?page_id=239355"},"modified":"2021-05-19T13:50:35","modified_gmt":"2021-05-19T13:50:35","slug":"fluorescence-in-situ-hybridization-fish-services","status":"publish","type":"page","link":"https:\/\/wolfson.net\/clgenetics\/services\/fluorescence-in-situ-hybridization-fish-services\/","title":{"rendered":"Fluorescence in Situ Hybridization (FISH) Services"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.9.3&#8243; background_color=&#8221;#f7f7f4&#8243; custom_padding=&#8221;||30px||false|false&#8221;][et_pb_row column_structure=&#8221;3_4,1_4&#8243; _builder_version=&#8221;4.9.3&#8243; background_size=&#8221;initial&#8221; background_position=&#8221;top_left&#8221; background_repeat=&#8221;repeat&#8221; custom_margin=&#8221;0px||0px||false|false&#8221; custom_padding=&#8221;0px||0px||false|false&#8221; locked=&#8221;off&#8221;][et_pb_column type=&#8221;3_4&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_text _builder_version=&#8221;4.9.3&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;|800||on|||||&#8221; header_text_color=&#8221;#4595cf&#8221; header_font_size=&#8221;40px&#8221; custom_margin=&#8221;||30px||false|false&#8221; animation_style=&#8221;fade&#8221; animation_delay=&#8221;500ms&#8221;]<\/p>\n<h1>Fluorescence in Situ Hybridization (FISH) Services<\/h1>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;2_3,1_3&#8243; _builder_version=&#8221;4.6.6&#8243; background_size=&#8221;initial&#8221; background_position=&#8221;top_left&#8221; background_repeat=&#8221;repeat&#8221; custom_margin=&#8221;0px||0px||false|false&#8221; custom_padding=&#8221;0px||0px||false|false&#8221; locked=&#8221;off&#8221;][et_pb_column type=&#8221;2_3&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_text ul_item_indent=&#8221;5%&#8221; _builder_version=&#8221;4.9.4&#8243; _module_preset=&#8221;default&#8221; text_font_size=&#8221;16px&#8221; ul_font=&#8221;||||||||&#8221; ul_font_size=&#8221;12px&#8221; ul_line_height=&#8221;1.8em&#8221; header_font=&#8221;|800||on|||||&#8221; header_text_color=&#8221;#4595cf&#8221; header_font_size=&#8221;40px&#8221; custom_margin=&#8221;||30px|||&#8221; animation_style=&#8221;fade&#8221; animation_delay=&#8221;900ms&#8221;]<\/p>\n<p>FISH is a sensitive and useful tool for detecting small populations of interphase and metaphase cells with clonal aberrations. Once a clonal aberration is identified by routine karyotyping or aCGH, FISH probes targeting the same aberration can be used on earlier passages of the cell line to determine whether earlier passages are aberration-free. FISH can be used track longitudinal cytogenetic changes in cancer cells. Because of its high degree of sensitivity, FISH can also be used to screen large numbers of cultures for recurrent chromosome aberrations and can be used to detect recurrent aberrations in cell lines that otherwise appear normal by standard cytogenetics.<\/p>\n<p><strong>What is Fluorescence in Situ Hybridization (FISH)?<\/strong><br \/>FISH involves tagging DNA of interest with complementary fluorescent probes that can be documented with fluorescent microscopy.<\/p>\n<p>Cell Line Genetics offers standard FISH services as well as an array of highly customizable options such as designing specific genetic probes or verification of cells that have been CRISPR-based gene edited.<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_gallery gallery_ids=&#8221;25235,25234&#8243; fullwidth=&#8221;on&#8221; _builder_version=&#8221;4.9.4&#8243; _module_preset=&#8221;default&#8221; animation_style=&#8221;fade&#8221; animation_delay=&#8221;900ms&#8221; auto=&#8221;on&#8221;][\/et_pb_gallery][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.6.6&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#efefe9&#8243; min_height=&#8221;20px&#8221; height=&#8221;20px&#8221; max_height=&#8221;20px&#8221; custom_margin=&#8221;0px||0px||false|false&#8221; custom_padding=&#8221;0px||0px||false|false&#8221; locked=&#8221;off&#8221;][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.9.2&#8243; background_color=&#8221;#f7f7f4&#8243; custom_margin=&#8221;||0px||false|false&#8221; custom_padding=&#8221;||0px||false|false&#8221;][et_pb_row _builder_version=&#8221;4.9.3&#8243; background_size=&#8221;initial&#8221; background_position=&#8221;top_left&#8221; background_repeat=&#8221;repeat&#8221; custom_margin=&#8221;0px||0px||false|false&#8221; custom_padding=&#8221;0px||0px||false|false&#8221; locked=&#8221;off&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.9.3&#8243; custom_padding=&#8221;||0px||false|false&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_text ul_item_indent=&#8221;5%&#8221; _builder_version=&#8221;4.9.4&#8243; _module_preset=&#8221;default&#8221; text_font_size=&#8221;16px&#8221; ul_font=&#8221;||||||||&#8221; ul_font_size=&#8221;16px&#8221; ul_line_height=&#8221;1.8em&#8221; header_font=&#8221;|800||on|||||&#8221; header_text_color=&#8221;#4595cf&#8221; header_font_size=&#8221;40px&#8221; custom_margin=&#8221;||-20px||false|false&#8221; custom_padding=&#8221;||0px||false|false&#8221; animation_style=&#8221;fade&#8221; animation_delay=&#8221;900ms&#8221;]<\/p>\n<h2><strong>Services:<\/strong><\/h2>\n<ul>\n<li><strong>Standard FISH services: <\/strong>Used to identify small emerging abnormal clones, complex chromosome aberrations, small partial trisomies, small marker chromosomes, and to refine chromosome breakpoints.\n<ul>\n<li>CLG offers FISH services for a large variety of genes and chromosome regions, including CLG StemCheckTM Probes\n<ul>\n<li><span><a href=\"http:\/\/wolfson.net\/clgenetics\/samples\/\">Complete Probe List<\/a><\/span><\/li>\n<\/ul>\n<\/li>\n<li>The CLG Human StemCheckTM FISH probes provide users with a quick and economic means to assess the occurrence of trisomy within their cultured Stem Cell lines.\n<ul>\n<li>The StemCheckTM FISH probes target the 4 most common trisomies (Chr 1,12,17,20) that can arise during the culturing and passaging of ESC and iPSC lines.<\/li>\n<li>CLG\u2019s Critical 4 (human) were identified by leveraging our library of Stem Cell Abnormalities obtained through the evaluation of more than 17,000 cultured human iPSC and ESC lines.<\/li>\n<li>Since FISH analysis provides a cell-by-cell perspective of the overall cell population (single cell), users of the StemCheckTM FISH Probes will be able to evaluate for mixed populations.<\/li>\n<\/ul>\n<\/li>\n<li>CLG offers gene specific FISH probes for rapid determination of gene copy number.\n<ul>\n<li>Some genes offered include PTEN, BCL2L1, EGFR, RET, etc.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Complimentary aCGH verification: <\/strong>Confirm aCGH duplications and deletions using fluorescently labeled probes.\n<ul>\n<li>CLG uses fluorescence in situ hybridization (FISH) to confirm array comparative genomic hybridization (aCGH) findings. CLG can confirm genomic amplifications and deletions along with detecting the location of the aberrations that are larger than 40KB in size.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Transgene integration: <\/strong>Determine the chromosome band location of your inserted transgene.\n<ul>\n<li>CLG can use fluorescence in situ hybridization (FISH) to confirm the integration of transgene and determine their chromosome band location.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Gene editing verification<\/strong><\/li>\n<li><strong>Custom FISH probe design, localization, and QA: <\/strong>Can&#8217;t find the probe you are looking for? CLG can provide specific FISH probes to meet your research need.\n<ul>\n<li>Probes for specific genes (larger than 40kb), chromosomes bands, and specific chromosome regions.<\/li>\n<li>CLG has the FISH expertise to work with difficult research samples, including cancer and gene-edited cell lines.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>[\/et_pb_text][dvmd_table_maker _builder_version=&#8221;4.9.4&#8243; _module_preset=&#8221;default&#8221; tbl_chead_cell_align_vert=&#8221;center&#8221; tbl_chead_cell_align_horz=&#8221;center&#8221; tbl_chead_cell_color=&#8221;#EFEFE9&#8243; tbl_chead_text_font=&#8221;|700|||||||&#8221; tbl_stripes_active=&#8221;on&#8221; hover_enabled=&#8221;0&#8243; sticky_enabled=&#8221;0&#8243; tbl_column_max_width=&#8221;500px&#8221; module_alignment=&#8221;center&#8221; tbl_column_min_width=&#8221;500px&#8221; tbl_scrolling_active=&#8221;on&#8221; tbl_scrolling_col_sticky=&#8221;on&#8221; width=&#8221;80%&#8221;][dvmd_table_maker_item _builder_version=&#8221;4.9.4&#8243; 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_builder_version=&#8221;4.9.4&#8243; _dynamic_attributes=&#8221;button_url&#8221; _module_preset=&#8221;default&#8221; custom_button=&#8221;on&#8221; button_text_size=&#8221;16px&#8221; button_text_color=&#8221;#FFFFFF&#8221; button_bg_color=&#8221;#4595CF&#8221; button_border_color=&#8221;RGBA(0,0,0,0)&#8221; button_font=&#8221;|700||on|||||&#8221; button_use_icon=&#8221;off&#8221; custom_margin=&#8221;0px||20px||false|false&#8221; custom_padding=&#8221;10px|50px|10px|50px|false|false&#8221; global_module=&#8221;239450&#8243; saved_tabs=&#8221;all&#8221;][\/et_pb_button][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;Tab_Style_26&#8243; _builder_version=&#8221;4.9.4&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#F7F7F4&#8243; custom_margin=&#8221;0px||||false|false&#8221; custom_padding=&#8221;0px||||false|false&#8221;][et_pb_row admin_label=&#8221;TAB MODULE TEMPLATE 26&#8243; _builder_version=&#8221;4.5.6&#8243; _module_preset=&#8221;default&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.5.6&#8243; _module_preset=&#8221;default&#8221; custom_css_main_element_last_edited=&#8221;on|tablet&#8221; custom_css_main_element_tablet=&#8221;||&#8221;][et_pb_tabs active_tab_background_color=&#8221;#434343&#8243; inactive_tab_background_color=&#8221;#D7D7D7&#8243; active_tab_text_color=&#8221;#ffffff&#8221; admin_label=&#8221;Style 26&#8243; module_class=&#8221;et_pb_tabs_dct_3_26&#8243; _builder_version=&#8221;4.9.4&#8243; _module_preset=&#8221;default&#8221; tab_text_color=&#8221;#808080&#8243; body_text_align=&#8221;justify&#8221; body_text_color=&#8221;#808080&#8243; body_letter_spacing=&#8221;0.2px&#8221; body_line_height=&#8221;30px&#8221; custom_css_tabs_controls=&#8221;position: relative;||background: none;||margin-bottom: 8px;||perspective: 17em;&#8221; custom_css_tab=&#8221;margin-right: 10px;||border: 0px solid #9b9da0 ;||||&#8221; custom_css_tabs_content=&#8221;text-transform: capitalize;||border: 2px solid #9b9da0 ;||height: 100%;&#8221; border_width_all=&#8221;0px&#8221; locked=&#8221;off&#8221; tab_text_color__hover_enabled=&#8221;on|hover&#8221; tab_text_color__hover=&#8221;#ffffff&#8221; inactive_tab_background_color__hover_enabled=&#8221;on|desktop&#8221; inactive_tab_background_color__hover=&#8221;#4595CF&#8221; custom_css_tab_last_edited=&#8221;on|desktop&#8221; custom_css_tab_tablet=&#8221;display: grid;||margin-right: 0px;||margin-bottom: 10px;||border-bottom: none;||width: 100%;||border-right:none;&#8221; active_tab_background_color__hover_enabled=&#8221;on|hover&#8221;][et_pb_tab title=&#8221;Attributes&#8221; _builder_version=&#8221;4.9.4&#8243; _module_preset=&#8221;default&#8221; custom_css_after=&#8221;border: none;&#8221;]<\/p>\n<ul>\n<li>Harvesting of live cell cultures for FISH analysis.<\/li>\n<li>Fixed cell pellets and frozen cell pellets also accepted.<\/li>\n<li>Interphase Nuclei analysis of 200 cells per cell line. Metaphase analysis also available.<\/li>\n<li>High quality images in publication ready format.<\/li>\n<li>Comprehensive interpretation of results by a CLG scientist.<\/li>\n<li>Report signed by clinically certified cytogeneticist.<\/li>\n<li>Results securely stored in CLG database.<\/li>\n<li>7-10 day turn around \u2013 <strong>Rush services are available.<\/strong><strong><\/strong><\/li>\n<\/ul>\n<p>[\/et_pb_tab][et_pb_tab title=&#8221;Service Specs&#8221; _builder_version=&#8221;4.9.4&#8243; _module_preset=&#8221;default&#8221; custom_css_after=&#8221;border: none;&#8221;]<\/p>\n<table style=\"border-collapse: collapse; width: 100%;\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"width: 20%;\"><strong>\u00a0<\/strong><\/td>\n<td style=\"width: 79.88560533841755%; text-align: center;\"><strong>Standard FISH Services<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 20%;\"><strong>Used to:<\/strong><\/td>\n<td style=\"width: 79.88560533841755%;\">\n<ul>\n<li>As an adjunct to G-Band Karyotyping:<\/li>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>To identify complex chromosome aberrations<\/li>\n<li>To identify small partial trisomies<\/li>\n<li>To identify small marker chromosomes<\/li>\n<li>To refine chromosome breakpoints<\/li>\n<li>\n<div><span lang=\"EN-CA\">To detect small emerging abnormal clones<\/span><\/div>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"border-collapse: collapse; width: 100%;\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"width: 20%;\"><b><span lang=\"EN-CA\">Detects:<\/span><\/b><\/td>\n<td style=\"width: 79.88560533841755%;\">\n<ul>\n<li>Genomic sequences of interest:<\/li>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>Duplications and deletions &gt;40KB<\/li>\n<li>Low level mosaicism<\/li>\n<li>Unbalanced translocations<\/li>\n<li>Cryptic chromosome aberrations<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"border-collapse: collapse; width: 100%;\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"width: 20%;\">\n<div><b><span lang=\"EN-CA\">Doesn\u2019t Detect:<\/span><\/b><\/div>\n<\/td>\n<td style=\"width: 79.88560533841755%;\">\n<ul>\n<li>\n<div><span lang=\"EN-CA\">Chromosome aberrations other than the probe sequence of interest<\/span><\/div>\n<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"border-collapse: collapse; width: 100%;\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"width: 20%;\">\n<div><b><span lang=\"EN-CA\">Sample Requirements:<\/span><\/b><\/div>\n<\/td>\n<td style=\"width: 79.88560533841755%;\">\n<ul>\n<li>Live Cell Culture (T-25 Flask)<\/li>\n<li>Frozen Cells<\/li>\n<li><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"border-collapse: collapse; width: 100%;\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"width: 20%;\">\n<div><b><span lang=\"EN-CA\">Species:<\/span><\/b><\/div>\n<\/td>\n<td style=\"width: 79.88560533841755%;\">\n<ul>\n<li>Human<\/li>\n<li>Mouse<\/li>\n<li>Contact us about other species<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"border-collapse: collapse; width: 100%;\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"width: 20%;\">\n<div><b><span lang=\"EN-CA\">Turnaround:<\/span><\/b><\/div>\n<\/td>\n<td style=\"width: 79.88560533841755%;\">\n<ul>\n<li>\n<div><span lang=\"EN-CA\">7 &#8211; 10 business days <\/span><\/div>\n<\/li>\n<li>\n<div><span lang=\"EN-CA\"><b>RUSH services available<\/b><\/span><\/div>\n<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/et_pb_tab][et_pb_tab title=&#8221;Sample Requirements&#8221; _builder_version=&#8221;4.9.4&#8243; _module_preset=&#8221;default&#8221; custom_css_after=&#8221;border: none;&#8221;]<\/p>\n<p><strong>PROCEDURE: <\/strong><span lang=\"EN-CA\"><a href=\"http:\/\/wolfson.net\/clgenetics\/wp-content\/uploads\/2021\/05\/Mailing-Live-Cultures-2.12.16.pdf\" target=\"_blank\" rel=\"noopener\"><span>Mailing Live Cultures For FISH<\/span><\/a><\/span><\/p>\n<div><\/div>\n<p><strong>Requisition Form<\/strong><br \/>Each sample must be accompanied by its own Test Requisition Form. Fill out a requisition form <a title=\"Request for Cell Line Characterization\" href=\"http:\/\/wolfson.net\/clgenetics\/samples\/\">here<\/a>.<\/p>\n<p>[\/et_pb_tab][et_pb_tab title=&#8221;References&#8221; _builder_version=&#8221;4.9.4&#8243; _module_preset=&#8221;default&#8221; body_ol_item_indent=&#8221;5px&#8221; body_ol_text_align=&#8221;left&#8221; body_ol_line_height=&#8221;1.5em&#8221; custom_css_after=&#8221;border: none;&#8221;]<\/p>\n<ol>\n<ol>\n<ol>\n<li>Merz, M., Jauch, A., Hielscher, T., Mai, E. K., Seckinger, A., Hose, D., Bertsch, U., Neben, K., Raab, M. S., Salwender, H., Blau, I. W., Lindemann, H. W., Schmidt-Wolf, I., Scheid, C., Haenel, M., Weisel, K., Goldschmidt, H., &amp; Hillengass, J. (2017). Longitudinal fluorescence\u00a0<em>in situ<\/em>hybridization reveals cytogenetic evolution in myeloma relapsing after autologous transplantation.\u00a0<em>Haematologica<\/em>,\u00a0<em>102<\/em>(8), 1432\u20131438. <a href=\"https:\/\/doi.org\/10.3324\/haematol.2017.168005\">https:\/\/doi.org\/10.3324\/haematol.2017.168005<\/a><\/li>\n<\/ol>\n<\/ol>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol>\n<ol>\n<li>Shakoori A. R. (2017). Fluorescence In Situ Hybridization (FISH) and Its Applications.\u00a0<em>Chromosome Structure and Aberrations<\/em>, 343\u2013367. <a href=\"https:\/\/doi.org\/10.1007\/978-81-322-3673-3_16\">https:\/\/doi.org\/10.1007\/978-81-322-3673-3_16<\/a><\/li>\n<\/ol>\n<\/ol>\n<p>[\/et_pb_tab][\/et_pb_tabs][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=&#8221;4.5.3&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||0px||false|false&#8221; custom_padding=&#8221;||0px||false|false&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.5.3&#8243; _module_preset=&#8221;default&#8221;][et_pb_text disabled_on=&#8221;on|on|on&#8221; admin_label=&#8221;CSS Is Located In A Code Module Under This Text&#8221; _builder_version=&#8221;4.9.2&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;|600||on|||||&#8221; header_2_font=&#8221;|600||on|||||&#8221; text_orientation=&#8221;center&#8221; disabled=&#8221;on&#8221;]<\/p>\n<p>CSS is located in a code module under this text<\/p>\n<p>[\/et_pb_text][et_pb_code admin_label=&#8221;TEMPLATE 26  Style 26 CSS&#8221; _builder_version=&#8221;4.9.2&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221;]<\/p>\n<style><!-- [et_pb_line_break_holder] -->.et_pb_tabs_dct_3_26 .et_pb_tabs_controls::after {<!-- [et_pb_line_break_holder] -->\tborder: none;<!-- [et_pb_line_break_holder] -->}<!-- [et_pb_line_break_holder] -->.et_pb_tabs_dct_3_26 .et_pb_tabs_controls li a {<!-- [et_pb_line_break_holder] -->\toutline: none;<!-- [et_pb_line_break_holder] -->\tpadding: 8px 12px;<!-- [et_pb_line_break_holder] -->\tposition: relative;<!-- [et_pb_line_break_holder] -->\toverflow: hidden;<!-- [et_pb_line_break_holder] -->\tz-index: 1;<!-- [et_pb_line_break_holder] -->\ttext-transform: uppercase;<!-- [et_pb_line_break_holder] -->\tborder: 1px solid transparent;<!-- [et_pb_line_break_holder] -->\ttext-align:center;<!-- [et_pb_line_break_holder] -->}<!-- [et_pb_line_break_holder] -->.et_pb_tabs_dct_3_26 .et_pb_tabs_controls li a:before,<!-- [et_pb_line_break_holder] -->.et_pb_tabs_dct_3_26 .et_pb_tabs_controls li a:after {<!-- [et_pb_line_break_holder] -->\tcontent: \"\";<!-- [et_pb_line_break_holder] -->\twidth: 100%;<!-- [et_pb_line_break_holder] -->\theight: 50%;<!-- [et_pb_line_break_holder] -->\tbackground: rgba(0, 0, 0, 0.5);<!-- [et_pb_line_break_holder] -->\tposition: absolute;<!-- [et_pb_line_break_holder] -->\ttop: 0;<!-- [et_pb_line_break_holder] -->\tleft: 0;<!-- [et_pb_line_break_holder] -->\tz-index: -1;<!-- [et_pb_line_break_holder] -->\ttransform-origin: 100% 0;<!-- [et_pb_line_break_holder] -->\ttransform: rotateZ(90deg);<!-- [et_pb_line_break_holder] -->\ttransition: all 0.3s ease 0s;<!-- [et_pb_line_break_holder] -->}<!-- [et_pb_line_break_holder] --><!-- [et_pb_line_break_holder] -->.et_pb_tabs_dct_3_26 .et_pb_tabs_controls li a:after {<!-- [et_pb_line_break_holder] -->\ttop: auto;<!-- [et_pb_line_break_holder] -->\tbottom: 0;<!-- [et_pb_line_break_holder] -->\ttransform-origin: 0 100%;<!-- [et_pb_line_break_holder] -->}<!-- [et_pb_line_break_holder] --><!-- [et_pb_line_break_holder] -->.et_pb_tabs_dct_3_26 .et_pb_tabs_controls li a:hover:before,<!-- [et_pb_line_break_holder] -->.et_pb_tabs_dct_3_26 .et_pb_tabs_controls li a:hover:after {<!-- [et_pb_line_break_holder] -->\ttransform: rotateZ(0);<!-- [et_pb_line_break_holder] -->}<!-- [et_pb_line_break_holder] --><\/style>\n<p>[\/et_pb_code][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fluorescence in Situ Hybridization (FISH) ServicesFISH is a sensitive and useful tool for detecting small populations of interphase and metaphase cells with clonal aberrations. Once a clonal aberration is identified by routine karyotyping or aCGH, FISH probes targeting the same aberration can be used on earlier passages of the cell line to determine whether earlier [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":90,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_et_pb_use_builder":"on","_et_pb_old_content":"<!-- wp:paragraph -->\r\n<p>Cell Line Genetics (CLG) is recognized world-wide for its expertise in cell line karyotyping analysis. Unlike most cytogenetic core facilities and commercial laboratories focused on clinical testing, CLG is dedicated to providing cell therapy and regenerative medicine researchers with the highest quality cytogenetics service available with fast turn-around guaranteed.<\/p>\r\n<p>What is Karyotyping?<\/p>\r\n<p><PLACE CONTENT HERE. INCLUDE PICTURE><\/p>\r\n<p>Cell Line Genetics' scientists are clinically trained and experienced in cell genomic stability analysis and structural chromosomal anomalies unique to various types of cell lines\u2014many of which can lead to invasive cell populations and other adverse genetic events. Since the company\u2019s inception, Cell Line Genetics has characterized thousands of research and therapeutic cell lines including:<\/p>\r\n<ul>\r\n<li>Stem Cells (Embryonic, Induced Pluripotent, Hematopoietic)<\/li>\r\n<li>Primary Cells\u00a0<\/li>\r\n<li>CAR-T Cells\u00a0<\/li>\r\n<li>N\/K Cells\u00a0<\/li>\r\n<li>Gene-Edited Cells (CRISP-Cas9)<\/li>\r\n<li>Multiple Species (Human, Mouse, Rat, Rhesus)<\/li>\r\n<\/ul>\r\n<p>Feel free to contact us at <page><\/p>\r\n<p><Attributes> <Service Specs> <Sample Requirements><\/p>\r\n<p>\u00a0<\/p>\r\n<!-- \/wp:paragraph -->","_et_gb_content_width":"1080","inline_featured_image":false,"footnotes":""},"class_list":["post-239355","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Fluorescence in Situ Hybridization (FISH) Services -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/wolfson.net\/clgenetics\/services\/fluorescence-in-situ-hybridization-fish-services\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Fluorescence in Situ Hybridization (FISH) Services -\" \/>\n<meta property=\"og:description\" content=\"Fluorescence in Situ Hybridization (FISH) ServicesFISH is a sensitive and useful tool for detecting small populations of interphase and metaphase cells with clonal aberrations. 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