Array Comparative Genomic Hybridization (aCGH)

Array Comparative Genomic Hybridization (aCGH) is a high-resolution karyotype analysis solution for the detection of unbalanced structural and numerical chromosomal alterations with high-throughput capabilities. Addition of SNPs can also detect polyploidy, loss of heterozygosity, and uniparental dysomy. It is especially useful as it can detect these aberrations at such a high resolution and on the genome level. The test also generates quantitative data which can be used for computational biology.

What is Array Comparative Genomic Hybridization (aCGH)?
Array Comparative Genomic Hybridization is used to determine relative copy number variations between a test sample and reference genomic DNA. Reference or control DNA samples are fluor-labeled in one color, and the DNA of interest is labeled with another color. The two genomes are co-hybridized onto an array of DNA fragments. The relative signal intensity correlates to copy number ratio. Although, aCGH gives relative information on copy number between the genome of interest and the references genome, FISH can further be utilized to determine exact copy number in a region of interest.

CLG StemCheck™ FISH Probe 1/20

CLG offers two different array comparative genomic hybridization arrays to help characterize your cell lines. CLG utilizes Agilent’s Microarray platform. Complementary FISH validation services for aCGH studies are available. (See FISH services for further information).

Services:

  • SurePrint G3 Human CGH Microarray Kit, 8x60K: High-throughput, low-cost alternative to karyotyping. Average coverage every 41KB with increased coverage in Refseq genes (33KB)
  • SurePrint G3 Human Genome CGH+SNP Microarray Kit, 4x180K: High resolution array with SNPs included. Average coverage every 25KB with increased coverage in ISCA regions (5KB)
Submit Samples for Testing
SurePrint G3 Human CGH Microarray Kit, 8x60K
 SurePrint G3 Human Genome CGH+SNP Microarray Kit, 4X180K
  • Arrays contain optimized, validated probes, and internal quality control features.
  • 55,077 distinct genomic probes.
  • 41 kb median probe spacing.
  • 60K probes.
  • 1000 replicates of biological probes.
  • Graphical and quantitative data included in report.
  • Results securely stored in CLG database.
  • 7-10 day turn around – Rush services are available.
  • Research Use Only (RUO) or Good Laboratory Practices (GLP) services are available.
  • Arrays contain optimized, validated probes, and internal quality control features.
  • 110,712 (CGH) and 59,647 (SNP) distinct genomic probes.
  • 25.3 kb median probe spacing.
  • 180K probes.
  • 3000 replicates of biological probes.
  • Graphical and quantitative data included in report Results securely stored in CLG database.
  • 7-10 day turn around – Rush services are available.
  • Research Use Only (RUO) or Good Laboratory Practices (GLP) services are available.
  Standard FISH Services
Used to:
  • As an adjunct to G-Band Karyotyping:
    • To identify complex chromosome aberrations
    • To identify small partial trisomies
    • To identify small marker chromosomes
    • To refine chromosome breakpoints
    • To detect small emerging abnormal clones
Detects:
  • Genomic sequences of interest:
    • Duplications and deletions >40KB
    • Low level mosaicism
    • Unbalanced translocations
    • Cryptic chromosome aberrations
Doesn’t Detect:
  • Chromosome aberrations other than the probe sequence of interest
Sample Requirements:
  • Live Cell Culture (T-25 Flask)
  • Frozen Cells
Species:
  • Human
  • Mouse
  • Contact us about other species
Turnaround:
  • 7 – 10 business days
  • RUSH services available

PROCEDURE: Mailing Live Cultures For FISH

Requisition Form
Each sample must be accompanied by its own Test Requisition Form. Fill out a requisition form here.

  1. Merz, M., Jauch, A., Hielscher, T., Mai, E. K., Seckinger, A., Hose, D., Bertsch, U., Neben, K., Raab, M. S., Salwender, H., Blau, I. W., Lindemann, H. W., Schmidt-Wolf, I., Scheid, C., Haenel, M., Weisel, K., Goldschmidt, H., & Hillengass, J. (2017). Longitudinal fluorescence in situhybridization reveals cytogenetic evolution in myeloma relapsing after autologous transplantation. Haematologica, 102(8), 1432–1438. doi.org/10.3324/haematol.2017.168005
  2. Shakoori A. R. (2017). Fluorescence In Situ Hybridization (FISH) and Its Applications. Chromosome Structure and Aberrations, 343–367. doi.org/10.1007/978-81-322-3673-3_16